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Reagents and Materials Sterile Krebs-Ringer Buffer (KRB) (See Section 2.1) KRB with 1 mM EDTA (See Section 2.2) Collagenase, 0.055% (w/v) (See Section 2.3) Autoclaved surgical instruments: partial curve microdissecting forceps, rat tooth tweezers, scalpel, ne sharp-tip scissors, and Mayo blunt scissors Cotton-tipped applicators Lengths of 3-0 silk suture, 6 in., 2 Petri dishes Nonsterile Rat, Female Lewis, 5 8 weeks old, 1 Perfusion system: Water bath to warm perfusate to 39 C out ow temperature and 95% O2 -5% CO2 cylinder to equilibrate perfusate through semipermeable tubing. The end point is attached to a sterile 18-gauge Angiocath catheter Veterinary-grade iso urane anesthesia device (Model 100F) Betadine Ethanol, 70% Beaker, 1 L with lid Hair clippers Vacuum Surgical tray (perforated) with collection tub Protocol (a) Flush perfusion reservoir and tubing with 70% ethanol, then 500 ml UPW, and then 100 ml KRB-EDTA. Add 400 ml KRB-EDTA, clear bubbles from line, and close the perfusion circuit. Set ow rate to 20 ml/min. Allow 15 min for warming and equilibration of KRB-EDTA. (b) Anesthetize rat with vaporizer: Adjust ow rate on the anesthesia device to 1.0 L/min 95% O2 -5% CO2 , and set the iso urane level to 5% v/v. Put exit tubing from the device with nose-cone end in a large beaker and cover, allowing a few minutes for the concentration of iso urane to build. Transfer rat into the beaker, cover, and monitor until fully sedated. (c) After initial sedation, remove rat from beaker and position on paper towels with nose-cone covering its mouth. Maintain portable anesthesia, with iso urane set to 2.5% v/v. Shave abdomen fur and vacuum loose fur. (d) Transfer rat to surgical tray with collection tub underneath, and tape limbs to restrain. Swab abdominal region generously with ethanol, then Betadine.

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In the Color Swatches panel, choose one of the following commands from the pop-up menu in the upper right corner:

Uncomment them to the following:

If you need to remove the content or ActionScript from a keyframe but still need the frame for timing purposes, choose Insert Clear Keyframe. If you need to convert several standard frames to keyframes, select the frames and then choose Modify Frames Convert to Keyframes. To convert a selection of frames to blank keyframes, choose Modify Frames Convert to Blank Keyframes.

In this approach, validation is performed after the execution of the transaction but just before the transaction is committed. If validation fails, transaction will be aborted. It should be obvious that the cost of abort in this case is the highest since validation is performed last. Consequently, the lock duration for the data items with which a transaction is working is also long.

1

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IP DATACAST (IPDC)

# lvcreate --name music --size 3G VGforMyth # mkreiserfs /dev/VGforMyth/music # mount /dev/VGforMyth/music /var/music

Language Family Baltic Basque Celtic Celtic Celtic Celtic Dravidian Dravidian Dravidian Dravidian Eskimo Eskimo Finno-Ugric Finno-Ugric Finno-Ugric Germanic Germanic Germanic Germanic Germanic Germanic Germanic Germanic Germanic Germanic Germanic Hamitic Hamitic Hamitic Ibero-Caucasian Ibero-Caucasian

Figure 10-9. (Continued)

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travertine spring A spring (q.v.) which precipitates travertine (q.v.). tributyl tin oxide (TBTO) An arti cial organic compound of tin, long used as a component of anti-fouling paints in the maritime industry. trophic chains Predation hierarchies in ecosystems with one animal being eaten by another, which is in turn eaten by others etc. uncon ned aquifer An aquifer (q.v.) in which the upper limit of saturation (q.v.) (neglecting the capillary fringe (q.v.)) is the water table (q.v.), such that unsaturated soil or sub-soil lies between the upper boundary of the aquifer and the ground surface. underground thermal energy storage (UTES) The introduction of heat into the subsurface (either with downhole heat exchanges or by injection of warmed water), whence it can later be removed during a period of high heat demand. undersaturated The geochemical condition in which the ionic activities of speci ed dissolved species do not exceed the thermodynamic thresholds above which the water would be likely to begin precipitating a given mineral. uniformitarianism The principle that observation of present-day processes is the key to understanding past events, as evident in the geological record. unsaturated zone Those parts of the subsurface in which pores are only partly lled with water, and thus contain a mixture of air and soil moisture (q.v.). urban heat island An urban area which has a warmer climate than surrounding rural areas, due to the disposal of waste heat from multiple sources. vadose Obsolete term meaning unsaturated (as in unsaturated zone (q.v.)).

H (Xi |Xi 1 , . . . , X1 , Y )

to the range of a stream while discussing the PLAY method. In RTSP, the stream range is speci ed using the Range request header; we discuss some of the RTSP message headers in next section.

There are three categories of optical ber, as distinguished by their modal and physical properties:

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