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Sensors locations play a critical role in numerous sensor network applications. For example, target tracking applications require sensors locations to estimate the moving direction of target objects. As another example, in geographical routing protocols (e.g., FACE [31,32], greedy perimeter stateless routing (GPSR) [32 34]), sensor nodes make routing decisions at least partially based on their own and their neighbors locations. Indeed, many sensor network applications will not work without sensors location information. A number of location discovery protocols [33,35 42] have been proposed for wireless sensor networks in recent years. These protocols share a common feature: They all use some special nodes, called beacon nodes, which are assumed to know their own locations (e.g., through global positioning system (GPS) receivers or manual con guration). These protocols work in two stages. In the rst stage, nonbeacon nodes receive radio signals called beacon signals from the beacon nodes. The packet carried by a beacon signal, which we call a beacon packet, usually includes the location of the beacon node. The nonbeacon nodes then estimate certain measurements (e.g., distance between the beacon and the nonbeacon nodes) based on features of the beacon signals (e.g., received signal strength indicator, time difference of arrival). We refer to such a measurement and the location of the corresponding beacon node collectively as a location reference. In the second stage, a sensor node determines its own location when it has enough number-of-location references

Reagents and Materials Nonsterile Paraformaldehyde, 4% Ethanol, 70% Reagents for embedding Safranin-O/Fast Green stain Histostain-SP kit Protocol (a) Observe the hydrogels by inverted light microscopy during incubation. In general, the cells may be observed and their distribution in the gel can be monitored. Also, opacity that develops in the gel, indicative of matrix formation, can be observed over time. (b) After the required culture period, harvest the constructs and x overnight in 4% paraformaldehyde at 4 C. (c) After the overnight incubation, transfer the samples to 70% ethanol until embedded in paraf n. Section to 5 m.

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neighborhood and makes a request for the phone company to disconnect the phone service and remove the listing the next time the phonebooks are updated. Registration and deregistration can be done in many different ways. They can use a centralized service running on a single machine, they can involve several services deployed throughout the architecture, or they can even be done with broadcast messages to all COS instances currently running in the environment. Obviously, each of these approaches has advantages and disadvantages. A centralized approach could become a bottleneck for communication and could be costly for COS instances deployed on faraway machines. This mechanism is also somewhat wasteful, since each COS instance probably does not need to communicate with every other COS instance in the architecture. The broadcast mechanism is similarly wasteful and costly with respect to bandwidth and potential timeouts in communication. Using some form of Registration Service deployed for a local region of the network is the more typical implementation, because it can provide a reasonable amount of control, with minimal overhead. These local registries can communicate with each other to synchronize information on a regularly scheduled or need-to-know basis, similar to how Domain Name Servers (DNS) work in a TCP/IP network.

3. If the consumer is participating in the program, the card associa-

N OT E Most of the programs I have covered run on the Windows platform; however, for the remainder of this chapter, all the tools I cover are Linux-based.

Within each of these major systems are nested structure-process components, whereby the intrapsychic is the most microscopic and the sociocultural-family the most macroscopic, each level being subsumed by the other in what Bronfenbrenner (1969) described as nested Russian dolls in his landmark volume, The Ecology of Developmental Process: Experiments by Nature and Design. Germain (1991) wrote: